How to use this tool
- Enter starting concentration, transferred sample, diluent per step, number of steps.
- Select Calculate to view the result.
- Check the method and assumptions below before using the result.
The method, explained
Each step retains a fraction transfer ÷ (transfer + diluent) of the previous concentration. After n steps, concentration = initial concentration × fraction^n.
Using starting concentration = 1 mol/L, transferred sample = 1 mL, diluent per step = 9 mL, number of steps = 4, the result is 0.0001 mol/L. Change these example inputs to match your task; use the method above to check each step.
Understanding your result
Here a tenfold dilution uses one part sample plus nine parts diluent, making ten parts total. The displayed factor follows total volume divided by sample volume.
What to keep in mind
Assumes full mixing between steps and the same transfer and diluent volumes each time. Educational calculation only; use validated procedures for laboratory work.
Reference: OpenStax Chemistry 2e — chemical quantities and models
Common questions
Does a 1:10 dilution mean one part sample plus ten parts water?
Here a tenfold dilution uses one part sample plus nine parts diluent, making ten parts total. The displayed factor follows total volume divided by sample volume.
Are my inputs uploaded?
No. This calculation runs in your browser without an account or an external API.
Methodology maintained by ClarityKit. How these tools are built and checked.